SAUER LAB
General Information
The lab uses biophysical, genetic, structural, and design strategies to study the relationship between the sequence, stability, and three-dimensional structures of proteins.
We are also interested in the mechanisms that intracellular proteases use to select the correct targets and how ATP-dependent proteases catalyze protein denaturation.
Selected Sauer Lab Publications
Barkow, S.R., Levchenko, I., Baker, T.A. & Sauer, R.T. (2009) Polypeptide translocation by the AAA+ ClpXP protease machine. Chemistry & Biology 16, 605-612.
Davis, J.H., Baker, T.A. & Sauer, R.T. (2009) Engineering synthetic adaptors and substrates for controlled ClpXP degradation. J. Biol. Chem., 21848-21855.
Sohn, J. & Sauer, R.T. (2009) OMP peptides modulate the activity of DegS protease by differential binding to active and inactive conformations. Mol. Cell 33, 64-74.
Moore, S.D., Baker, T.A. & Sauer, R.T. (2008) Forced extraction of targeted components from complex macromolecular assemblies. Proc. Natl. Acad. Sci. USA 105, 11685-11690.
Gur, E. & Sauer, R.T. (2008) Recognition of misfolded proteins by Lon, a AAA+ protease. Genes Dev. 22, 2267-2277.
Martin, A., Baker, T.A. & Sauer, R.T. (2008) Pore loops of the AAA+ ClpX machine grip substrates to drive translocation and unfolding. Nat. Struct. Mol. Biol. 15, 1147-1151.
Martin, A., Baker, T.A. & Sauer, R.T. (2008) Protein unfolding by a AAA+ protease: critical dependence on ATP-hydrolysis rates and energy landscapes. Nat. Struct. Mol. Biol. 15, 139-145.
Sohn, J., Grant, R.A. & Sauer, R.T. (2007) Allosteric activation of DegS, a stress sensor PDZ-protease. Cell 131, 572-583.
Cezairliyan, B.O. & Sauer, R.T. (2007) Inhibition of regulated proteolysis by RseB. Proc. Natl. Acad. Sci. USA 10, 3771-3776.
Farrell. C.M., Baker, T.A. & Sauer, R.T. (2007) Altered specificity of a AAA+ protease. Mol. Cell 25, 161-166.
McGinness, K.E., Baker, T.A. & Sauer, R.T. (2006) Engineering controllable protein degradation. Mol. Cell 22, 701-707.
Bolon, D.N., Grant, R.A., Baker, T.A. & Sauer, R.T. (2005) Specificity versus stability in computational protein design. Proc. Natl. Acad. Sci. USA 102, 12724-12729.
Martin, A., Baker, T.A. & Sauer, R.T. (2005) Rebuilt AAA+ motors reveal operating principles for ATP-fueled machines. Nature 437, 1115-1120.
Hersch, G.L., Burton, R.E., Bolon, D.N., Baker, T.A. & Sauer, R.T. (2005) Asymmetric interactions of ATP with the AAA+ ClpX6 unfoldase: allosteric control of a protein machine. Cell 121, 1017-1027.
Tabtiang, R.K., Cezairliyan, B.O., Grant, R.A, Cochrane, J.C. & Sauer, R.T. (2005) Consolidating critical binding determinants by non-cyclic rearrangement of protein secondary structure. Proc. Natl. Acad. Sci. USA 102, 2305-2309.
Burton, R.E., Baker, T.A. & Sauer, R.T. (2005) Nucleotide-dependent substrate recognition by the AAA+ HslUV protease. Nat. Struct. & Mol. Biol. 12, 245-251.
Joshi, S.A., Hersch, G.L., Baker, T.A. & Sauer, R.T. Communication between ClpX and ClpP during substrate processing and degradation. Nature Structural & Molecular Biology 11, 404-411 (2004).
Bolon, D.N., Wah, D.A., Hersch, G.L., Baker, T.A. & Sauer, R.T. Bivalent tethering of SspB to ClpXP is required for efficient substrate delivery: a protein-design study. Molecular Cell 13, 443-449 (2004).
Hayes, C.S. & Sauer, R.T. Cleavage of the A-site mRNA codon during ribosome pausing provides a mechanism for translational quality control. Molecular Cell 12, 903-911 (2003).
Kenniston, J.A., Baker, T.A., Fernandez, J.M. & Sauer, R.T. Linkage between ATP consumption and mechanical unfolding during the protein processing reactions of an AAA+ degradation machine. Cell 114, 511-520 (2003).
Walsh, N.P., Alba, B.M., Bose, B., Gross, C.A. & Sauer, R.T. OMP peptide signals initiate the envelope-stress response by activating DegS protease through relief of inhibitory interactions mediated by its PDZ domain. Cell 113, 61-71 (2003).
Cordes, M.H.J., Burton, R.E., Walsh, N.P., McKnight, C.J. & Sauer, R.T. An Evolutionary Bridge to a New Protein Fold. Nature Structural Biology 7, 1129-1132 (2000).
The Links
Sauer Lab, Massachusetts Insitute of Technology
77 Massachusetts Avenue, 68-571
Cambridge, MA 02139
Telephone: 617.253.6077, Fax: 617.258.0673To locate us, please use the following url: http://whereis.mit.edu/map-jpg
Contact: Webmaster as follows: sauer-www (at) mit.edu
(Email format (at) = @)Copyright 2006