LOCUS Optimized\pcyA 842 bp DNA linear 15-JUN-2009 DEFINITION Synthesis Plan. SOURCE ORGANISM COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|521114559| COMMENT VNTDBDATE|521139694| COMMENT LSOWNER| COMMENT VNTNAME|Optimized pcyA| COMMENT VNTAUTHORNAME|Cory| COMMENT VNTEXTCHREPL|Yeast COMMENT Vector_NTI_Display_Data_(Do_Not_Edit!) 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(CObjectList)) COMMENT #184=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -1157 100) COMMENT (CObjectList) (CObjectList))) (CObjectList))) COMMENT (CSeqView 10 10 (CObjectList) (CObList) 1 (CObList)) (CObList) 38601128 COMMENT (CStringList) 0 16625072 (CObList))) FEATURES Location/Qualifiers CDS 37..810 /vntifkey="4" /label=pcyA misc_feature 819..839 /vntifkey="21" /label=Biobrick\Suffix RBS 26..36 /vntifkey="32" /label=Kozak misc_feature 4..25 /vntifkey="21" /label=Biobrick\Prefix misc_feature 37..63 /vntifkey="21" /label=MTS BASE COUNT 243 a 128 c 177 g 294 t ORIGIN 1 gcggaattcg cggccgcttc tagagctcga gaacatatgc aacgctccat ttttgcgagg 61 ttcgctgtta ctgatttgtc tttgactaat tcttctttga tgccaacttt gaatccaatg 121 attcaacaat tggctttggc tattgctgct tcttggcaat ctttgccatt gaaaccatat 181 caattgccag aagatttggg ttatgttgaa ggtagattgg aaggtgaaaa attggttatt 241 gaaaatagat gttatcaaac tccacaattt agaaaaatgc atttggaatt ggctaaagtt 301 ggtaaaggtt tggatatttt gcattgtgtt atgtttccag aaccattgta tggtttgcca 361 ttgtttggtt gtgatattgt tgctggtcca ggtggtgttt ctgctgctat tgctgatttg 421 tctccaactc aatctgatag acaattgcca gctgcttatc aaaaatcttt ggctgaattg 481 ggtcaaccag aatttgaaca acaaagagaa ttgccaccat ggggtgaaat tttttctgaa 541 tattgtttgt ttattagacc atctaatgtt actgaagaag aaagatttgt tcaaagagtt 601 gttgattttt tgcaaattca ttgtcatcaa tctattgttg ctgaaccatt gtctgaagct 661 caaactttgg aacatagaca aggtcaaatt cattattgtc aacaacaaca aaaaaatgat 721 aaaactagaa gagttttgga aaaagctttt ggtgaagctt gggctgaaag atatatgtct 781 caagttttgt ttgatgttat tcaataataa ttaattaata ctagtagcgg ccgctgcagg 841 cg //