LOCUS YCp22FL1 7383 bp DNA circular 8-APR-2009 SOURCE ORGANISM COMMENT Koloteva, N, P P Müller, and J E McCarthy. “The position dependence of translational regulation via RNA-RNA and RNA-protein interactions in the 5'-untranslated region of eukaryotic mRNA is a function of the thermodynamic competence of 40 S ribosomes in translational initiation.” The Journal of Biological Chemistry 272, no. 26 (June 27, 1997): 16531-9. Vector sent by McCarthy COMMENT This file is created by Vector NTI http://www.invitrogen.com/ COMMENT VNTDATE|514996375| COMMENT VNTDBDATE|515004527| COMMENT LSOWNER| COMMENT VNTNAME|YCp22FL1| COMMENT VNTAUTHORNAME|Brian| COMMENT VNTAUTHOREML|bbelmont@mit.edu| FEATURES Location/Qualifiers promoter 1..591 /vntifkey="29" /label=TEF1\Promoter misc_feature 592..632 /vntifkey="21" /label=MCS CDS 633..2285 /vntifkey="4" /label=Firefly\Luciferase terminator 2286..2562 /vntifkey="43" /label=PGK1\Terminator centromere 2811..3787 /vntifkey="5" /label=CEN4 rep_origin 3788..4422 /vntifkey="33" /label=ARS1 CDS complement(4422..5064) /vntifkey="4" /label=TRP1 CDS 5350..6210 /vntifkey="4" /label=AmpR rep_origin 6968..6968 /vntifkey="33" /label=pBR322\ori misc_feature 7..2587 /vntifkey="21" /label=Sequenced primer_bind 7308..7328 /vntifkey="28" /label=OL-106:\YCp22FL\seq\1 primer_bind 509..529 /vntifkey="28" /label=OL-107:\YCp22FL\seq\2 primer_bind complement(2643..2663) /vntifkey="28" /label=OL-110:\YCp33SUPEX/22FL\seq\3 misc_feature 598..598 /vntifkey="21" /label=transcription\initiation\site BASE COUNT 2085 a 1609 c 1535 g 2154 t ORIGIN 1 tcccagtcac gacgttgtaa aacgacggcc agtgctacaa tcgcggccgc ataggtatcg 61 atcgtcaccc tgcagggtga cggatcgatc cgtcacccgc atattacata taatacatat 121 cacataggaa gcaacaggcg cgttggactt ttaattttcg aggaccgcga atccttacat 181 cacacccaat cccccacaag tgatccccca cacaccatag cttcaaaatg tttctactcc 241 ttttttactc ttccagattt tctcggactc cgcgcatcgc cgtaccactt caaaacaccc 301 aagcacagca tactaaattt cccctctttc ttcctctagg gtgtcgttaa ttacccgtac 361 taaaggtttg gaaaagaaaa aagagaccgc ctcgtttctt tttcttcgtc gaaaaaggca 421 ataaaaattt ttatcacgtt tctttttctt gaaaattttt ttttttgatt tttttctctt 481 tcgatgacct cccattgata tttaagttaa taaacggtct tcaatttctc aagtttcagt 541 ttcatttttc ttgttctatt acaacttttt ttacttcttg ttcattagaa aggatccaat 601 tatctactta agaacacaaa actcgagaac atatggaaga cgccaaaaac ataaagaaag 661 gcccggcgcc attctatcca ctagaggatg gaaccgctgg agagcaactg cataaggcta 721 tgaagagata cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtga 781 acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat 841 atgggctgaa tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta 901 tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata 961 atgaacgtga attgctcaac agtatgaaca tttcgcagcc taccgtagtg tttgtttcca 1021 aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt accaataatc cagaaaatta 1081 ttatcatgga ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat 1141 ctcatctacc tcccggtttt aatgaatacg attttgtacc agagtccttt gatcgtgaca 1201 aaacaattgc actgataatg aattcctctg gatctactgg gttacctaag ggtgtggccc 1261 ttccgcatag aactgcctgc gtcagattct cgcatgccag agatcctatt tttggcaatc 1321 aaatcattcc ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt 1381 ttactacact cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag 1441 aagagctgtt tttacgatcc cttcaggatt acaaaattca aagtgcgttg ctagtaccaa 1501 ccctattttc attcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac 1561 acgaaattgc ttctgggggc gcacctcttt cgaaagaagt cggggaagcg gttgcaaaac 1621 gcttccatct tccagggata cgacaaggat atgggctcac tgagactaca tcagctattc 1681 tgattacacc cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg 1741 aagcgaaggt tgtggatctg gataccggga aaacgctggg cgttaatcag agaggcgaat 1801 tatgtgtcag aggacctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg 1861 ccttgattga caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg 1921 aacacttctt catagttgac cgcttgaagt ctttaattaa atacaaagga tatcaggtgg 1981 cccccgctga attggaatcg atattgttac aacaccccaa catcttcgac gcgggcgtgg 2041 caggtcttcc cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg 2101 gaaagacgat gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga 2161 aaaagttgcg cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac 2221 tcgacgcaag aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagtccaaat 2281 tgtaatctag aattccttcg atagatcaat ttttttcttt tctctttccc catcctttac 2341 gctaaaataa tagtttattt tattttttga atatttttta tttatatacg tatatataga 2401 ctattattta tcttttaatg attattaaga tttttattaa aaaaaaattc gctcctcttt 2461 taatgccttt atgcagtttt tttttcccat tcgatatttc tatgttcggg ttcagcgtat 2521 tttaagttta ataactcgaa aattctgcgt tcgttaaagc ttggcgtaat catggtcata 2581 gctgttactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact 2641 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac 2701 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga tgcggtattt 2761 tctccttacg catctgtgcg gtatttcaca ccgcatatat cgctgggcca ttctcatgaa 2821 gaatatcttg aatttattgt catattacta gttggtgtgg aagtccatat atcggtgatc 2881 aatatagtgg ttgacatgct ggctagtcaa cattgagcct tttgatcatg caaatatatt 2941 acggtatttt acaatcaaat atcaaactta actattgact ttataactta tttaggtggt 3001 aacattctta taaaaaagaa aaaaattact gcaaaacagt actagctttt aacttgtatc 3061 ctaggttatc tatgctgtct caccatagag aatattacct atttcagaat gtatgtccat 3121 gattcgccgg gtaaatacat ataatacaca aatctggctt aataaagtct ataatatatc 3181 tcataaagaa gtgctaaatt ggctagtgct atatattttt aagaaaattt cttttgacta 3241 agtccatatc gactttgtaa aagttcactt tagcatacat atattacacg agccagaaat 3301 tgtaactttt gcctaaaatc acaaattgca aaatttaatt gcttgcaaaa ggtcacatgc 3361 ttataatcaa cttttttaaa aatttaaaat acttttttat tttttatttt taaacataaa 3421 tgaaataatt tatttattgt ttatgattac cgaaacataa aacctgctca agaaaaagaa 3481 actgttttgt ccttggaaaa aaagcactac ctaggagcgg ccaaaatgcc gaggctttca 3541 tagcttaaac tctttacaga aaataggcat tatagatcag ttcgagtttt cttattcttc 3601 cttccggttt tatcgtcaca gttttacagt aaataagtat cacctcttag agttcgatga 3661 taagctgtca aacatgagaa ttaattccac atgttaaaat agtgaaggag catgttcggc 3721 acacagtgga ccgaacgtgg ggtaagtgca ctagggtccg gttaaacgga tctcgcattg 3781 atgaggcaac gctaattatc aacatataga ttgttatcta tctgcatgaa cacgaaatct 3841 ttacttgacg acttgaggct gatggtgttt atgcaaagaa accactgtgt ttaatatgtg 3901 tcactgtttg atattactgt cagcgtagaa gataatagta aaagcggtta ataagtgtat 3961 ttgagataag tgtgataaag tttttacagc gaaaagacga taaatacaag aaaatgatta 4021 cgaggatacg gagagaggta tgtacatgtg tatttatata ctaagctgcc ggcggttgtt 4081 tgcaagaccg agaaaaggct agcaagaatc gggtcattgt agcgtatgcg cctgtgaaca 4141 ttctcttcaa caagtttgat tccattgcgg tgaaatggta aaagtcaacc ccctgcgatg 4201 tatattttcc tgtacaatca atcaaaaagc caaatgattt agcattatct ttacatcttg 4261 ttattttaca gattttatgt ttagatcttt tatgcttgct tttcaaaagg cttgcaggca 4321 agtgcacaaa caatacttaa ataaatacta ctcagtaata acctatttct tagcattttt 4381 gacgaaattt gctattttgt tagagtcttt tacaccattt gtctccacac ctccgcttac 4441 atcaacacca ataacgccat ttaatctaag cgcatcacca acattttctg gcgtcagtcc 4501 accagctaac ataaaatgta agctctcggg gctctcttgc cttccaaccc agtcagaaat 4561 cgagttccaa tccaaaagtt cacctgtccc acctgcttct gaatcaaaca agggaataaa 4621 cgaatgaggt ttctgtgaag ctgcactgag tagtatgttg cagtcttttg gaaatacgag 4681 tcttttaata actggcaaac cgaggaactc ttggtattct tgccacgact catctccatg 4741 cagttggacg atatcaatgc cgtaatcatt gaccagagcc aaaacatcct ccttaggttg 4801 attacgaaac acgccaacca agtatttcgg agtgcctgaa ctatttttat atgcttttac 4861 aagacttgaa attttccttg caataaccgg gtcaattgtt ctctttctat tgggcacaca 4921 tataataccc agcaagtcag catcggaatc tagtgcacat tctgcggcct ctgtgctctg 4981 caagccgcaa actttcacca atggaccaga actacctgtg aaattaataa cagacatact 5041 ccaagctgcc tttgtgtgct taatcacgta tactcacgtg ctcaatagtc accaatgccc 5101 tccctcttgg ccctctcctt ttcttttttc gaccgaatta attcttgaag acgaaagggc 5161 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca 5221 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat 5281 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa 5341 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt 5401 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag 5461 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt 5521 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg 5581 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag 5641 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta 5701 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg 5761 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta 5821 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac 5881 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt 5941 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca 6001 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag 6061 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta 6121 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag 6181 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt 6241 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat 6301 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta 6361 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa 6421 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt 6481 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag 6541 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta 6601 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca 6661 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag 6721 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa 6781 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga 6841 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc 6901 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc 6961 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt 7021 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt 7081 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag 7141 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa 7201 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat 7261 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg 7321 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac 7381 gcc //